5OJ3

YCF48 from Cyanidioschyzon merolae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.12713
Spacegroup name	P 21 21 21
Unit cell lengths	60.810, 97.120, 110.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.690 - 2.982
R-factor	0.2255
Rwork	0.223
R-free	0.28010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xbg
RMSD bond length	0.004
RMSD bond angle	0.942
Data reduction software	xia2
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.690	3.030
High resolution limit [Å]	2.980	2.980
Rmerge	0.128	0.951
Rpim		0.542
Number of reflections	13864	688
<I/σ(I)>	10.6	1.6
Completeness [%]	99.7	99.7
Redundancy	3.9	4
CC(1/2)	0.993	0.537

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	10 % w/v PEG 1000/ 10 % w/v PEG 8000