5OIX
Structure of the HMPV P oligomerization domain at 1.6 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.000, 110.440, 38.730 |
| Unit cell angles | 90.00, 95.62, 90.00 |
Refinement procedure
| Resolution | 36.390 - 1.610 |
| Rwork | 0.179 |
| R-free | 0.20350 |
| Data reduction software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.390 |
| High resolution limit [Å] | 1.610 |
| Rmerge | 0.046 |
| Rpim | 0.029 |
| Number of reflections | 31091 |
| <I/σ(I)> | 13.89 |
| Completeness [%] | 99.3 |
| Redundancy | 3.3 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 20% polyethylene glycol (PEG) 6000, 200 mM NaCl, and 100 mM Tris, pH 8.0 |
