5OID

Complex Trichoplax STIL-NTD:human CEP85 coiled coil domain 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.93927
Spacegroup name	I 41 3 2
Unit cell lengths	268.699, 268.699, 268.699
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	95.000 - 4.600
R-factor	0.3533
Rwork	0.353
R-free	0.36510
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.896
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	SHELXDE
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.000	5.140
High resolution limit [Å]	4.600	4.600
Rmerge	0.272	3.033
Rpim	0.043	0.470
Number of reflections	9532	2642
<I/σ(I)>	13.2	1.9
Completeness [%]	100.0	100
Redundancy	39.9	42.1
CC(1/2)	0.999	0.397

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	100 mM MES, pH 6.0 1.45 M MgSO4 2 mM DTT