5OHX

Structure of active cystathionine B-synthase from Apis mellifera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-10-04
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.542
Spacegroup name	P 21 21 21
Unit cell lengths	86.143, 96.099, 180.684
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.360 - 3.200
R-factor	0.1986
Rwork	0.197
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pc2
RMSD bond length	0.003
RMSD bond angle	1.128
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400	3.300
High resolution limit [Å]	3.200	3.200
Number of reflections	25365
<I/σ(I)>	10.2	2.6
Completeness [%]	99.7
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	10% PEG 6000, 100 mM HEPES pH 7.5, 5% MPD