5OHQ

Crystal structure of the KOW6-KOW7 domain of human DSIF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999987357022
Spacegroup name	C 2 2 21
Unit cell lengths	35.091, 75.657, 96.749
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.374 - 1.098
R-factor	0.1233
Rwork	0.122
R-free	0.14010
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.163
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXDE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.375	48.375	1.160
High resolution limit [Å]	1.098	3.290	1.100
Rmerge	0.032	0.029	0.163
Rmeas	0.035	0.032	0.203
Total number of observations	574723
Number of reflections	93250	3763	9310
<I/σ(I)>	27.7	62.25	4.32
Completeness [%]	91.7	99.1	56.9
Redundancy	6.163	6.606	2.74
CC(1/2)	0.999	0.998	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M bis-tris propane pH 7, 3 M sodium formate