5OHP

Crystal structure of USP30 (C77A) in complex with Lys6-linked diubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96860
Spacegroup name	P 65 2 2
Unit cell lengths	117.504, 117.504, 138.842
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	82.077 - 2.800
R-factor	0.2176
Rwork	0.216
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ohk
RMSD bond length	0.005
RMSD bond angle	0.695
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11rc1_2513))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.080	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.170	0.998
Rpim	0.086	0.504
Number of reflections	14395	1402
<I/σ(I)>	6.99	1.83
Completeness [%]	99.2	99.86
Redundancy	4.7	4.8
CC(1/2)	0.990	0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.73 M sodium citrate, 0.1 M Hepes pH 7.0