5OHG
enolase in complex with RNase E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-11-01 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.683, 116.923, 107.776 |
| Unit cell angles | 90.00, 105.43, 90.00 |
Refinement procedure
| Resolution | 39.244 - 1.997 |
| R-factor | 0.1625 |
| Rwork | 0.161 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fym |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.097 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.090 |
| High resolution limit [Å] | 1.997 | 1.990 |
| Rmerge | 0.099 | 0.301 |
| Rpim | 0.061 | 0.191 |
| Number of reflections | 118999 | |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 98.6 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M potassium thiocyanate, 0.1 M Bis-Tris propane (pH 8.5) and 20% (w/v) PEG 3350 |
