5OH9

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Thiazolidine-2,4-dione

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	56.548, 59.914, 88.562
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.300 - 1.650
R-factor	0.1739
Rwork	0.172
R-free	0.21028
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v2y
RMSD bond length	0.013
RMSD bond angle	1.506
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.300	1.750
High resolution limit [Å]	1.650	1.650
Rmerge	0.060	0.550
Number of reflections	36957	5832
<I/σ(I)>	10.1	1.71
Completeness [%]	99.1	97.5
Redundancy	2.87	2.68
CC(1/2)	1.000	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M sodium acetate pH 4.6, 15 %(W/V) PEG 20000