5OH5

Legionella pneumophila RidL N-terminal retromer binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-21
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	I 4 2 2
Unit cell lengths	110.140, 110.140, 98.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.255 - 1.900
R-factor	0.2045
Rwork	0.204
R-free	0.21360
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.840
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.255	49.255	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.053	0.020	1.880
Rmeas	0.055	0.021	1.954
Number of reflections	24155	324	1735
<I/σ(I)>	29.54	106.81	1.48
Completeness [%]	100.0	99.4	99.9
Redundancy	12.919	11.722	13.342
CC(1/2)	1.000	0.999	0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2M Magnesium chloride hexahydrate, 0.1M Tris, 20% (w/v) PEG 8000