5OH4

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Piperidine-2,6-dione (Glutarimide)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-09-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	56.180, 59.661, 88.289
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.100 - 2.300
R-factor	0.19228
Rwork	0.189
R-free	0.25008
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v2y
RMSD bond length	0.012
RMSD bond angle	1.463
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.100	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.080	0.370
Number of reflections	13628	2102
<I/σ(I)>	8.64	1.98
Completeness [%]	97.0	94
Redundancy	2.55	2.52
CC(1/2)	1.000	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M sodium acetate pH 4.6, 15 %(W/V) PEG 20000