5OGZ
Crystal structure of Ruminiclostridium Thermocellum beta-Glucosidase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.670, 56.220, 94.500 |
| Unit cell angles | 90.00, 100.20, 90.00 |
Refinement procedure
| Resolution | 93.010 - 1.600 |
| R-factor | 0.18438 |
| Rwork | 0.183 |
| R-free | 0.21612 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qox |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.021 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 93.010 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 120756 |
| <I/σ(I)> | 20.7 |
| Completeness [%] | 94.6 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291.15 | Lithium sulfate monohydrate, BIS-TRIS, Polyethylene glycol 3,350 |
