5OGX

Crystal structure of Amycolatopsis cytochrome P450 reductase GcoB.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	60.846, 65.903, 97.847
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.923 - 1.720
R-factor	0.1665
Rwork	0.165
R-free	0.19320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.079
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.850	1.750
High resolution limit [Å]	1.720	1.720
Rmerge	0.056	0.536
Rpim	0.036	0.353
Number of reflections	42590	2228
<I/σ(I)>	14.3	2.7
Completeness [%]	100.0	100
Redundancy	6.3	6.2
CC(1/2)	0.999	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		279	0.03M NaF, 0.03M NaBr, 0.03M NaI, 0.1M TRIS Bicine pH 8.5, 28% PEG500 MME, 14% PEG20000