5OFZ
PllA lectin, apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97779 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 92.107, 92.107, 164.182 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.054 - 1.750 |
| R-factor | 0.1948 |
| Rwork | 0.194 |
| R-free | 0.21310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l7l |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.999 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.054 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.047 | |
| Rmeas | 0.409 | |
| Rpim | 0.186 | |
| Number of reflections | 81583 | |
| <I/σ(I)> | 8.1 | 3.7 |
| Completeness [%] | 99.6 | 97.8 |
| Redundancy | 6.1 | 4.7 |
| CC(1/2) | 0.915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.6M Sodium citrate tribasic dihydrate pH 6.5 |
