5OFJ
Crystal structure of N-terminal domain of bifunctional CbXyn10C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 183.836, 183.836, 56.128 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.918 - 1.340 |
| R-factor | 0.1638 |
| Rwork | 0.163 |
| R-free | 0.18290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q8x |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.007 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2776: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.000 | 1.370 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Number of reflections | 106971 | 7803 |
| <I/σ(I)> | 12.7 | 1.7 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 12.7 | 10.7 |
| CC(1/2) | 0.990 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 16% (w/v) PEG20000 0.1 M Sodium citrate at pH 5.0 |
