5OF3

Crystal structure of the heterotrimeric PriSLX primase from S. solfataricus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 41
Unit cell lengths	104.889, 104.889, 229.758
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.910 - 2.906
R-factor	0.2305
Rwork	0.228
R-free	0.27130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zt2
RMSD bond length	0.006
RMSD bond angle	0.534
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.910	3.010
High resolution limit [Å]	2.906	2.906
Rmerge	0.105	1.404
Rpim	0.036	0.495
Number of reflections	54380	5392
<I/σ(I)>	15.09	1.47
Completeness [%]	100.0	99.93
Redundancy	9.3	9
CC(1/2)	0.998	0.527

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	11% PEG 8000, 21.5% ethylene glycol, 0.1 M bicine/Trizma base pH 8.5, 0.03 M di-, tri-, tetra- and penta-ethyleneglycol (Morpheus crystallization screen, Molecular Dimensions).