5OF2
The structural versatility of TasA in B. subtilis biofilm formation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91841 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.658, 43.039, 51.693 |
Unit cell angles | 90.00, 90.17, 90.00 |
Refinement procedure
Resolution | 41.658 - 1.860 |
R-factor | 0.1747 |
Rwork | 0.173 |
R-free | 0.21180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5of1 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.988 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.660 | 1.920 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.083 | 0.745 |
Number of reflections | 15615 | 1542 |
<I/σ(I)> | 13.83 | 1.85 |
Completeness [%] | 99.6 | 97.84 |
Redundancy | 4.5 | 4.4 |
CC(1/2) | 0.998 | 0.804 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293.15 | 34% PEG 2000 MME, 0.1M ammonium sulfate, 0.1M sodium acetate pH 4.8 |