5OEV
The structure of a glutathione synthetase like-effector (GSS22) from Globodera pallida in apoform.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.917 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.258, 122.393, 132.769 |
| Unit cell angles | 90.00, 97.61, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.180 |
| R-factor | 0.2163 |
| Rwork | 0.215 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kal |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.263 |
| Data reduction software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.180 | 2.200 |
| Rmerge | 0.070 | 0.839 |
| Rmeas | 0.076 | 0.903 |
| Number of reflections | 99955 | |
| <I/σ(I)> | 17 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293.15 | 0.2 M tri-methylamine N-oxide, 0.1 M Tris pH 9 and 20 % w/v PEG 2000 MME |
