5OET
The structure of a glutathione synthetase like-effector (GSS30) from Globodera pallida in apoform.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.750, 81.619, 93.840 |
| Unit cell angles | 90.00, 103.18, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.570 |
| R-factor | 0.2529 |
| Rwork | 0.251 |
| R-free | 0.28520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oev |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.482 |
| Data reduction software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.720 |
| High resolution limit [Å] | 2.570 | 2.600 |
| Rmerge | 0.136 | 0.997 |
| Rmeas | 0.149 | 1.097 |
| Number of reflections | 32990 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M magnesium formate di-hydrate and 20 % PEG 3350 |
