5OC6
Crystal structure of human tRNA-dihydrouridine(20) synthase dsRBD in complex with a 22 nucleotide dsRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98010 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 58.440, 115.560, 66.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.650 - 3.200 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wft |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.850 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.650 | 3.314 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.306 | |
| Number of reflections | 3855 | 358 |
| <I/σ(I)> | 5.11 | 0.85 |
| Completeness [%] | 97.5 | 93.44 |
| Redundancy | 5.8 | 3.8 |
| CC(1/2) | 0.995 | 0.302 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 15% v/v isopropanol 20 mM MgCl2 50 mM MES |
