5OC0
Structure of E. coli superoxide oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2012-09-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97973 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.046, 91.102, 97.923 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.870 - 1.970 |
| R-factor | 0.18845 |
| Rwork | 0.188 |
| R-free | 0.20437 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.829 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.090 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmeas | 0.103 | 1.212 |
| Number of reflections | 21862 | |
| <I/σ(I)> | 14.38 | 2 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 13 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | HEPES, glycerol, PEG 2000 MME, magnesium chloride |
