5OBY
Mycoplasma genitalium DnaK-NBD in complex with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9495 |
| Spacegroup name | H 3 |
| Unit cell lengths | 157.065, 157.065, 56.782 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 78.530 - 1.300 |
| R-factor | 0.142 |
| Rwork | 0.141 |
| R-free | 0.16140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5obw |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.132 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.730 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.500 | 0.580 |
| Rpim | 0.300 | 0.360 |
| Number of reflections | 127585 | 18174 |
| <I/σ(I)> | 12 | 1.9 |
| Completeness [%] | 99.5 | 97 |
| Redundancy | 7.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | Tris-HCl, ammonium sulfate, potassium thiocyanate |
