5OBW
Mycoplasma genitalium DnaK-NBD in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 |
| Unit cell lengths | 157.916, 157.916, 57.504 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 78.960 - 1.400 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.19730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5obu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.449 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.960 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.057 | 0.069 |
| Rpim | 0.028 | 0.032 |
| Number of reflections | 98799 | 6849 |
| <I/σ(I)> | 11.3 | 1.5 |
| Completeness [%] | 99.1 | 92 |
| Redundancy | 5.5 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Tris-HCl, ammonium sulfate, potassium thiocyanate |
