5OBV
Mycoplasma genitalium DnaK deletion mutant lacking SBDalpha in complex with ADP and Pi.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 133.547, 133.547, 64.519 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.430 - 2.490 |
| R-factor | 0.2182 |
| Rwork | 0.216 |
| R-free | 0.26560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5obu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.345 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.430 | 2.630 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.050 | 0.360 |
| Rpim | 0.016 | 0.120 |
| Number of reflections | 20864 | 2917 |
| <I/σ(I)> | 17.2 | 1.2 |
| Completeness [%] | 99.7 | 98 |
| Redundancy | 7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Sodium citrate, PEG MME 5000 and butanol. |
