5OBU
Mycoplasma genitalium DnaK deletion mutant lacking SBDalpha in complex with AMPPNP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 133.823, 133.823, 64.126 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.310 - 2.000 |
| R-factor | 0.1724 |
| Rwork | 0.170 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v7y |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.252 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.310 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.160 |
| Rpim | 0.026 | 0.057 |
| Number of reflections | 41211 | 5787 |
| <I/σ(I)> | 5.6 | 4 |
| Completeness [%] | 99.6 | 97.3 |
| Redundancy | 12 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Sodium citrate, PEG MME 5000 and butanol. |
