5OAT
PINK1 structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.915, 116.736, 179.344 |
| Unit cell angles | 90.00, 94.29, 90.00 |
Refinement procedure
| Resolution | 178.840 - 2.780 |
| R-factor | 0.20661 |
| Rwork | 0.205 |
| R-free | 0.24619 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.826 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 178.840 | 2.830 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.085 | 1.224 |
| Number of reflections | 87762 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 285 | 200 mM MgCl2, 100 mM HEPES pH 7.0, 20% PEG 6000 |
