5OAQ

TEAD4 COMPLEXED WITH YAP PEPTIDE AND MYRISTATE (COVALENTLY BOUND)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99999
Spacegroup name	P 41 21 2
Unit cell lengths	59.012, 59.012, 159.559
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.750 - 1.950
R-factor	0.2304
Rwork	0.229
R-free	0.25210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kys
RMSD bond length	0.010
RMSD bond angle	1.255
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.750
High resolution limit [Å]	1.950	1.950
Rmerge	0.072
Rmeas	0.075
Number of reflections	21390
<I/σ(I)>	28.2
Completeness [%]	99.8
Redundancy	12.8
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	15% PEG 3350,2% tacsimate, 0.1M NaCitrate