5OA9

Human translation re-initiation complex containing eIF2D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	273
Detector technology	PIXEL
Collection date	2016-09-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	50.720, 50.720, 197.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.670 - 1.800
R-factor	0.193
Rwork	0.190
R-free	0.23100
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.829
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.350	1.910
High resolution limit [Å]	1.800	1.800
Rmerge	0.057	1.026
Number of reflections	24936
<I/σ(I)>	14.9
Completeness [%]	99.8	99.9
Redundancy	6.9
CC(1/2)	0.999	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	292	1:1 mix protein (50 mM HEPES-KOH pH 7.6, 150 mM KCl, 1 mM TCEP) with reservoir solution (100 mM HEPES-KOH pH 7.6, 25 mM sodium formate, 25 mM ammonium acetate, 25 mM sodium citrate tribasic, 25 mM sodium potassium tartrate, 10.7% PEG3350, 10.7% MPD, 10.7% PEG1000)