5O9L

Crystal structure of human Histamine-Releasing Factor (HRF/TCTP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976
Spacegroup name	P 1 21 1
Unit cell lengths	49.725, 58.952, 57.733
Unit cell angles	90.00, 99.41, 90.00

Refinement procedure

Resolution	24.528 - 1.750
R-factor	0.19
Rwork	0.188
R-free	0.22090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yz1
RMSD bond length	0.004
RMSD bond angle	0.632
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.530	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.124	1.085
Rpim	0.065	0.667
Number of reflections	33094	1827
<I/σ(I)>	11.7	1.8
Completeness [%]	99.3	97.3
Redundancy	4.5	3.4
CC(1/2)	0.988	0.342

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	0.1M MMT pH4.0 and 25% w/v PEG 1500