5O9I
Crystal structure of transcription factor IIB Mvu mini-intein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-14 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.96770 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.183, 67.245, 51.226 |
| Unit cell angles | 90.00, 102.43, 90.00 |
Refinement procedure
| Resolution | 56.700 - 2.500 |
| R-factor | 0.22444 |
| Rwork | 0.222 |
| R-free | 0.27847 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.957 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.700 | 2.670 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.083 | 1.011 |
| Number of reflections | 12418 | |
| <I/σ(I)> | 13.3 | 1.9 |
| Completeness [%] | 99.5 | 96.7 |
| Redundancy | 7.6 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | calcium chloride, PEG 3350 |
