5O94
X-ray structure of a zinc binding GB1 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-18 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 54.178, 54.178, 81.077 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.920 - 1.900 |
| R-factor | 0.20779 |
| Rwork | 0.206 |
| R-free | 0.23392 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1igd |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.161 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.920 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.086 | 0.838 |
| Number of reflections | 11371 | |
| <I/σ(I)> | 17.2 | 2.6 |
| Completeness [%] | 99.9 | 92.8 |
| Redundancy | 10.6 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 70% MPD, 200 mM ZnSO4, 50 mM NaCI, pH 4.5 |
