5O8Z
Conformational dynamism for DNA interaction in Salmonella typhimurium RcsB response regulator.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.392, 54.266, 54.986 |
| Unit cell angles | 62.60, 81.61, 79.66 |
Refinement procedure
| Resolution | 48.690 - 2.100 |
| R-factor | 0.21545 |
| Rwork | 0.213 |
| R-free | 0.26542 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a04 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.367 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.690 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.851 |
| Rmeas | 0.082 | 1.004 |
| Rpim | 0.031 | 0.370 |
| Number of reflections | 21487 | 1736 |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 97.8 | 96.4 |
| Redundancy | 7.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 29% Jeffamine ED2003 0.1M lithium sulfate Tris pH 8.5 |
