5O8X
The X-ray Structure of Catenated Lytic Transglycosylase SltB1 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9796 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.064, 116.358, 54.865 |
| Unit cell angles | 90.00, 118.41, 90.00 |
Refinement procedure
| Resolution | 48.258 - 2.500 |
| R-factor | 0.1798 |
| Rwork | 0.177 |
| R-free | 0.22920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.838 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.260 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 21993 |
| <I/σ(I)> | 11.4 |
| Completeness [%] | 99.9 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1M Na-citrate, 0.1M Na-cacodylate pH=6.5 |
