5O8W

CRYSTAL STRUCTURE ANALYSIS OF THE YEAST ELONGATION FACTOR COMPLEX EEF1A:EEF1BA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-03-01
Detector	MARRESEARCH
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	63.850, 91.810, 92.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.724 - 1.670
R-factor	0.1573
Rwork	0.156
R-free	0.18440
RMSD bond length	0.009
RMSD bond angle	0.970
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.730
High resolution limit [Å]	1.670	1.670
Rmerge	0.040	0.155
Number of reflections	63533
<I/σ(I)>	36.1
Completeness [%]	99.3	93.5
Redundancy	3.5	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	mmE 2000, Tris, Hepes, KCl, DTT, NaAzid