5O8K
Crystal structure of mammalian Rev7 in complex with Rev3 1875-1895
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-13 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.93340 |
| Spacegroup name | P 32 |
| Unit cell lengths | 69.014, 69.014, 42.868 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.000 - 1.800 |
| Rwork | 0.255 |
| R-free | 0.28880 |
| Starting model (for MR) | 3abd |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.119 | 0.322 |
| Rmeas | 0.139 | 0.362 |
| Rpim | 0.071 | 0.166 |
| Number of reflections | 20408 | 3080 |
| <I/σ(I)> | 7.3 | 3.5 |
| Completeness [%] | 96.4 | 99.6 |
| Redundancy | 4.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 25% PEG 2000 MME, 0.8M sodium formate, 0.1M tris-HCl pH 7.5 |
