5O8D

Mutant of class II CPD photolyase from Methanosarcina mazei - Y345F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979003
Spacegroup name	P 43 21 2
Unit cell lengths	69.930, 69.930, 245.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.350 - 2.000
R-factor	0.1772
Rwork	0.177
R-free	0.21760
RMSD bond length	0.006
RMSD bond angle	0.773
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	81.800
High resolution limit [Å]	2.000
Rmerge	0.200
Number of reflections	42288
<I/σ(I)>	5.7
Completeness [%]	99.8
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.5 M LITHIUM SULFATE, 7.5% (W/V) PEG 6000