5O8B
Difference-refined excited-state structure of rsEGFP2 1ps following 400nm-laser irradiation of the off-state.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2015-05-01 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 1.3 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.530, 62.850, 71.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.943 - 1.700 |
| R-factor | 0.2911 |
| Rwork | 0.290 |
| R-free | 0.31120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dtx |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.751 |
| Data reduction software | CrystFEL (0.6.1) |
| Data scaling software | CrystFEL (0.6.1) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2376: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 16.943 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 17970 |
| <I/σ(I)> | 9.35 |
| Completeness [%] | 95.0 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 8 | 293 | 2 M ammonium sulphate, 20 mM NaCl, 120 mM HEPES |
