5O79
Klebsiella pneumoniae OmpK36
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.316, 74.511, 90.814 |
| Unit cell angles | 90.00, 112.18, 90.00 |
Refinement procedure
| Resolution | 107.570 - 1.650 |
| R-factor | 0.14829 |
| Rwork | 0.146 |
| R-free | 0.18963 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1osm |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.588 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.570 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rpim | 0.039 | 0.607 |
| Number of reflections | 169933 | 8276 |
| <I/σ(I)> | 11.6 | 1.6 |
| Completeness [%] | 98.5 | 97.3 |
| Redundancy | 3.8 | 3.9 |
| CC(1/2) | 0.689 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.08 M Magnesium Acetate tetrahydrate , 0.1 M sodium citrate, 14% w/v 5000 PEG MME |
