5O71
Crystal structure of human USP25
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-14 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.9791 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 140.806, 140.806, 190.156 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.080 - 3.283 |
| R-factor | 0.20586 |
| Rwork | 0.202 |
| R-free | 0.27514 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.524 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.080 | 3.294 |
| High resolution limit [Å] | 3.283 | 3.283 |
| Rmerge | 0.068 | 0.984 |
| Rmeas | 0.078 | 1.181 |
| Rpim | 0.041 | 0.626 |
| Number of reflections | 14883 | 129 |
| <I/σ(I)> | 16.3 | 2.3 |
| Completeness [%] | 99.5 | 97.7 |
| Redundancy | 6.5 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 3350, sodium fluoride |
