5O6S
UbV.B4R, a dimeric ubiquitin variant binding to BIRC4 RING
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2016-04-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 48.213, 71.669, 118.033 |
| Unit cell angles | 90.00, 94.97, 90.00 |
Refinement procedure
| Resolution | 58.790 - 2.900 |
| R-factor | 0.263 |
| Rwork | 0.261 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.060 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.790 | 3.070 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.169 | 0.523 |
| Rpim | 0.167 | 0.513 |
| Number of reflections | 8941 | 1423 |
| <I/σ(I)> | 3.7 | |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 2.9 | 2.9 |
| CC(1/2) | 0.966 | 0.725 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 292 | (0.2 M Zinc acetate, 0.1 M phosphate-citrate pH 4.2, 40 % PEG 300 |
