5O6N
High pressure flash cooled concanavalin A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-06 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 61.260, 86.230, 89.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.228 - 1.350 |
R-factor | 0.1339 |
Rwork | 0.133 |
R-free | 0.16390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jbc |
RMSD bond length | 0.005 |
RMSD bond angle | 0.822 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.230 | |
High resolution limit [Å] | 1.350 | |
Rmerge | 0.044 | 0.381 |
Rmeas | 0.048 | 0.455 |
Number of reflections | 51608 | |
<I/σ(I)> | 21.36 | |
Completeness [%] | 98.7 | |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 10 mg/ml Concanavalin A in 0.025 M HEPES pH 7.0 were mixed in a 1:1 ratio with 6 % (w/v) PEG 8000 and 0.1 M Tris pH 8.5. |