5O6E
Structure of ScPif1 in complex with TTTGGGTT and ADP-AlF4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979150 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 150.605, 150.605, 136.339 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.214 - 3.345 |
| R-factor | 0.1822 |
| Rwork | 0.178 |
| R-free | 0.25650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o6b |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.392 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.214 | 3.460 |
| High resolution limit [Å] | 3.340 | 3.340 |
| Rmerge | 0.170 | 0.800 |
| Number of reflections | 25042 | 2024 |
| <I/σ(I)> | 17.23 | 2.49 |
| Completeness [%] | 92.7 | 78.99 |
| Redundancy | 7.2 | 10.9 |
| CC(1/2) | 0.990 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 0.1M Tris-HCl 0.2M Na2SO4 15% PEG5000-MME |
