5O6B
Structure of ScPif1 in complex with GGGTTTT and ADP-AlF4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979150 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.246, 88.373, 187.703 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.707 - 2.029 |
R-factor | 0.1947 |
Rwork | 0.193 |
R-free | 0.23790 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.990 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | autoSHARP |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.710 | 2.100 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.060 | 0.490 |
Number of reflections | 80210 | 7015 |
<I/σ(I)> | 17.01 | 2.23 |
Completeness [%] | 98.1 | 86.8 |
Redundancy | 6.3 | 3.1 |
CC(1/2) | 0.990 | 0.740 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | 0.1M Tris-HCl 0.2M Na2SO4 15% EPEG5000-MME |