5O5W

Molybdenum storage protein room-temperature structure determined by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	293
Detector technology	PIXEL
Collection date	2016-06-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 63 2 2
Unit cell lengths	117.350, 117.350, 233.350
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.200 - 1.700
Rwork	0.180
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f6t
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	CrystFEL (0.6.2)
Data scaling software	CrystFEL (0.6.2)
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.200
High resolution limit [Å]	1.700
Rmerge	0.110
Number of reflections	110141
<I/σ(I)>	5.29
Completeness [%]	100.0
Redundancy	765.4
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0,1 M Na-Citrat pH 5,6 1,4 M NH4H2PO4 20 % Glycerol