5O5Q

X-ray crystal structure of RapZ from Escherichia coli (P3221 space group)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-01
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 32 2 1
Unit cell lengths	91.540, 91.540, 352.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.770 - 3.250
R-factor	0.2204
Rwork	0.218
R-free	0.27290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.734
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2747: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.770	3.366
High resolution limit [Å]	3.250	3.250
Rmerge	0.086	0.762
Number of reflections	27399
<I/σ(I)>	13.37	2.56
Completeness [%]	97.6
Redundancy	4.67	4.77
CC(1/2)	0.998	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1 M MES,1 M ammonium sulfate, 0.01 M mercaptoethanol, 1%(v/v) glycerol pH 7.0