5O5I
Robo1 Ig5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 61 |
| Unit cell lengths | 80.820, 80.820, 26.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.410 - 3.010 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v9q |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS (2 November 2014) |
| Data scaling software | XSCALE (2 November 2014) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rpim | 0.148 | 0.798 |
| Number of reflections | 2082 | 1026 |
| <I/σ(I)> | 7.3 | 1.5 |
| Completeness [%] | 98.3 | 94.1 |
| Redundancy | 3 | |
| CC(1/2) | 0.980 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 25% PEG 3350, 100 mM Sodium acetate, |
