5O5G
Robo1 Ig1 to 4 crystal form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9395 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.156, 99.711, 238.673 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.340 - 3.030 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v9r |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS (1st March 2015) |
| Data scaling software | XSCALE (1st March 2015) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rpim | 0.130 | 0.447 |
| Number of reflections | 10679 | 1866 |
| <I/σ(I)> | 6.4 | 1.4 |
| Completeness [%] | 99.6 | 98.7 |
| Redundancy | 3.9 | 3.9 |
| CC(1/2) | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 6% PEG 4000, 100 mM Sodium citrate, 10 mM Calcium chloride |
