5O56
Glycogen Phosphorylase b in complex with 29b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION NOVA |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2017-02-28 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.548, 128.548, 116.141 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.560 - 2.450 |
| R-factor | 0.16766 |
| Rwork | 0.164 |
| R-free | 0.22921 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.213 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.560 | 2.560 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 35487 | 4119 |
| <I/σ(I)> | 11.1 | 1.3 |
| Completeness [%] | 98.1 | 94.8 |
| Redundancy | 4.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.8 | 289 | 10 mM BES buffer |
