5O54

Glycogen Phosphorylase b in complex with 29a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION NOVA
Temperature [K]	293
Detector technology	CCD
Collection date	2017-02-28
Detector	AGILENT ATLAS CCD
Wavelength(s)	1.5419
Spacegroup name	P 43 21 2
Unit cell lengths	127.862, 127.862, 115.731
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.910 - 2.450
R-factor	0.1462
Rwork	0.144
R-free	0.18706
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.009
RMSD bond angle	1.313
Data reduction software	CrysalisPro
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.910	2.550
High resolution limit [Å]	2.450	2.450
Number of reflections	33526	3345
<I/σ(I)>	17.5	2.9
Completeness [%]	94.2	84.9
Redundancy	10.1	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.8	289	10 mM BES buffer