5O3Z
Crystal structure of Sorbitol-6-Phosphate 2-dehydrogenase SrlD from Erwinia amylovora
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91730 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 120.707, 138.057, 199.442 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 113.510 - 1.840 |
| R-factor | 0.22384 |
| Rwork | 0.209 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1geg |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.881 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 113.510 | 1.950 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 284126 | |
| <I/σ(I)> | 9.3 | 2.2 |
| Completeness [%] | 99.0 | 97.6 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | 100 mM HEPES pH 7.5, 600 mM MgCl2, 17% PEG3350 (cryo 20% Ethylene glycol) |
