5O3S

Carbon regulatory PII-like protein SbtB from Synechocystis sp. 6803 in Apo state, hexagonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	P 63
Unit cell lengths	50.535, 50.535, 64.951
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.290 - 2.200
R-factor	0.23109
Rwork	0.226
R-free	0.26763
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5o3p
RMSD bond length	0.004
RMSD bond angle	0.741
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.290	2.330
High resolution limit [Å]	2.200	2.200
Rmerge	0.095	0.764
Number of reflections	4838	776
<I/σ(I)>	12.25	2.12
Completeness [%]	100.0	100
Redundancy	10.1	9.8
CC(1/2)	0.996	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293.15	Protein solution containing 19 mg/ml SbtB in 50 mM Tris.HCl (pH 8.0), 250 mM NaCl mixed 1:1 with reservoir solution composed of 0.2 M Mg chloride, 0.1 M Tris.HCl pH 8.5, and 30% (w/v) PEG 4000.